Next-generation sequencing (NGS) is a high-throughput DNA sequencing technology that allows for rapid and efficient sequencing of large amounts of genetic material. NGS has revolutionized genomics research by enabling scientists to sequence entire genomes, transcriptomes, and epigenomes at a fraction of the time and cost compared to traditional Sanger sequencing methods. This technology has been instrumental in advancing our understanding of complex biological processes, identifying genetic variations associated with diseases, and facilitating personalized medicine. NGS has a wide range of applications in fields such as genetics, genomics, oncology, microbiology, and evolutionary biology.